Chromatography
Size Exclusion Chromatography/
Gel Permeation Chromatography (SEC/GPC)
SEC is used to separate molecules based on size in solution.
Applications:
- Analysis of polymer molecular weight distributions.
- Measurement of absolute molecular weights using light scattering.
- Analysis of molecular size and polymer chain conformation in solution using a differential viscometer detector.
- Relative molecular weight measurements using refractive index or UV/vis absorbance detector.
- Molecular weight analysis of polymers in organic solvents.
- SEC in aqueous mobile phases for water-soluble polymers.
- Quantitative analysis of low molecular weight molecules such as antioxidants, light stabilizers, plasticizers, lubricants and spin finishes.
- Preparative SEC with fraction collection for deformulation and contamination analysis.