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Chromatography
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Size Exclusion Chromatography/Gel Permeation Chromatography (SEC/GPC) image

Chromatography


Size Exclusion Chromatography/
Gel Permeation Chromatography (SEC/GPC)


SEC is used to separate molecules based on size in solution.

Applications:


  • Analysis of polymer molecular weight distributions.
  • Measurement of absolute molecular weights using light scattering.
  • Analysis of molecular size and polymer chain conformation in solution using a differential viscometer detector.
  • Relative molecular weight measurements using refractive index or UV/vis absorbance detector.
  • Molecular weight analysis of polymers in organic solvents.
  • SEC in aqueous mobile phases for water-soluble polymers.
  • Quantitative analysis of low molecular weight molecules such as antioxidants, light stabilizers, plasticizers, lubricants and spin finishes.
  • Preparative SEC with fraction collection for deformulation and contamination analysis.


Work We've Done


Molecular Weight Analysis using Size Exclusion Chromatography (SEC)





  • Polyamides (Nylon 6, Nylon 6/6, Nylon 12)
  • Polyesters (PET, PBT)
  • Poly(lactic acid) and poly(glycolic acid)
  • Polycaprolactone
  • Cellulose
  • Hydroxypropylmethyl cellulose (HPMC)
  • Carboxymethyl cellulose
  • Nitrocellulose
  • Hyaluronic acid
  • Gelatin
  • Poly(vinyl alcohol)
  • Ethylene-vinyl alcohol copolymers (EVOH)
  • Polypeptides
  • Silicone polymers