About Transmission Electron Microscopy Analysis

A concentrated beam of electrons passes through a very thin sample.  Based on electron density the various areas of the sample interact with the beam differently.  The resulting image represents the gradient areas of electron density. 


 

  • Approaches

    Transmission Electron Microscopy Analysis is a great approach for documenting the phase distribution of copolymers and blends.

    Many samples have sufficient contrast in an as-prepared state.  However, to obtain sufficient contrast with some specimens, a staining technique is utilized.  For example, unsaturated components within a matrix are stained with ruthenium tetroxide.  This method will reveal the rubber phase distribution in a polystyrene matrix.

    Ultra thin specimens are prepared using a microtome or a cryo microtome on polymer pieces or embedded specimens.

  • Sample Considerations

    TEM specimens are typically very thin, approximately 0.1 microns in thickness.  Specimens are acquired from regions of the sample based on the goal you hope to achieve through TEM testing. 

    To discuss your specific sample considerations in detail please contact us.

  • Experience

    Our experience with Transmission Electron Microscopy Analysis includes:

    • Phase morphology
    • Dispersion of polymer blends
    • Material selection
    • Quality control
    • Failure analysis
    • Competitive product analysis
    • Cellular structure of dispersed phases
    • Nanoscale particle incorporation
    • Metalized layer coherence and thickness analysis in laminated films