If we were forced to describe our team in one word we would choose “curious.” That word describes our staff from the front office to the labs and all support or technical roles in between. You see, our curious mindset paired with strong educational backgrounds and industry experience yields creative and sound solutions for our clients. It also yields some really interesting science experiments, like the creation of transparent wood.
One of our interns recently came across a paper about a group of researchers that produced transparent wood. In the most basic sense this group of researchers removed the lignin from the wood and replaced it with a polymer that cures transparent. Of course, the process to achieve transparent wood was much more complicated than those few sentences suggest. Lab-based interns requested approval to replicate this experiment and attempt to create transparent wood.
Permission was granted for their “off-time” when they weren’t busy supporting our laboratories. They were also required to work with our lab safety manager. And thus, the process began.
We wanted this to mimic the discovery process used during a research endeavor so it was required that the interns kept detailed lab notebook pages, as if this was being done for a client. They also had discovery meetings to plan out the experiment, to include how to purchase supplies and prepare solutions.
- Balsa wood was sourced from a local craft supply store. During the sample preparation phase it was cut into 20 x 20 x 6 mm squares
- The wood samples were placed in a crystallization dish and put in oven at 110oC for at least 24 hours for drying. This was necessary because the residual moisture in the wood must be taken out.
- A 1 wt % sodium chlorite solution in an acetate buffer was prepared for delignification of the wood
A solution was made and used immediately. The overall solution used was an acetate buffer with 1 wt % of sodium chlorite. To make this solution we made a 1 normal sodium acetate solution, and a 1 normal acetic acid solution. We then added sodium chlorite to a beaker and added both of the previous solutions to the beaker.
- In order to achieve delignification, the samples were added to the prepared sodium chlorite solution, heated to 80oC, and kept at this temperature for 6 hours.
- The solution was poured off, and the delignified samples were rinsed with ethanol, acetone, and water. The rinsing was done to dissolve any remaining solution and ensured no damage was done to our samples in the following steps:
– Rinsed 3 times with Reverse Osmosis Delonzied water
– Rinsed 3 times with ethanol
– Rinsed 3 times with a 1:1 ratio solution of ethanol and acetone
– Rinsed 3 times with acetone
- Polymerized methyl methacrylate (PMMA) was used to fill the wood. This polymer was synthesized by combining 0.3 wt % azobisisobutyronitrile (AIBN) and methyl methacrylate monomer at a temperature of 75o
- After the PMMA achieved the desirable viscosity, which was gauged by any thickening of the fluid, it was added to a crystallization dish.
- The delignified balsa squares were then added to the dish containing the PMMA. The dish was then placed in a vacuum oven for polymer infiltration. In other words, this is the step in which polymer was added to fill the gaps created when the lignin was removed.
- The vacuum oven was used to infiltrate the delignified balsa with the viscous polymer, in 30 minute intervals. After each interval, the vacuum was released.
- The samples were taken out of the vacuum oven, and sandwiched between glass slides.
- These slides were then wrapped in foil and put in the oven at 70oC for 4 hours
- After curing, the samples were then taken out of the foil and glass slides, and were ready for observation/testing
The resulting wood could be characterized as translucent rather than fully transparent. It is clearly different from untreated balsa wood, when doing a side by side comparison. It also has a glossy, smooth, and shiny finish as compared to the matte and rough finish of the untreated wood.
Image 1: From Left to Right, Virgin Balsa, Delignified Balsa, and PMMA Filled Balsa
Image 2: From Left to Right, Delignified Balsa Compared to Finished PMMA Filled Balsa
To refine the experimental process there are two portions of the experiment that could be modified in an effort to produce better results. First, a different method of delignification was identified in a separate research paper. Following this procedure could result in better delignification and therefore allow for more thorough polymer infiltration. Using microscopy techniques it would be possible to characterize the extent of the delignification and assess the effectiveness, compared to previous iterations, before proceeding to the polymer infiltration portion.
The other step in the process that could be revised is with the polymer infiltration. Based on images acquired with our Keyence Digital Microscope we can see that the wood samples were not fully infiltrated with PMMA. There are still open pores (Image 4) that show an incomplete infiltration. Future iterations could involve modifying the viscosity of the PMMA to see if it infiltrates better or could involve modifying the vacuum procedure to attempt to infiltrate better. These iterations would need to be carefully controlled and documented in order to create conditions that could be validated, after desired transparency is achieved. An Analytical technique that would be particularly relevant to refining this portion of the process would be digital microscopy, for assessing open pores. Our interns would tweak this experiment slightly, hoping to get a completely optically transparent wood
Image 3: Keyence Digital Microscopy Images of Radial Cuts of Delignified Balsa Wood
Image 4: Keyence Digital Microscopy Images of Radial Cuts of Filled Balsa Wood
*Note, there are still open pores, meaning not 100% PMMA fill
Why would we bother to spend time creating transparent wood with a team of interns? We aren’t in the construction business. We aren’t in the home décor business. We aren’t even a manufacturer of any sort. But what we are is a group that loves great science and a good challenge. That is exactly what this experimental process presented. It was a tremendous teachable moment for these up and coming scientists we are fortunate to have as interns. We know that when creativity and curiosity are allowed to flourish and are combined with the scientific process, discoveries are made. Discoveries about who we are, discoveries about science, and discoveries that improve the world we live in.
Zhu, M., Song, J., Li, T., Gong, A., Wang, Y., Dai, J., Yao, Y., Luo, W., Henderson, D. and Hu, L. (2016), Highly Anisotropic, Highly Transparent Wood Composites. Adv. Mater., 28: 5181–5187. doi:10.1002/adma.201600427
Yuanyuan Li, Qiliang Fu, Shun Yu, Min Yan, and Lars Berglund. (2016), Optically Transparent Wood from a Nanoporous Cellulosic Template: Combining Functional and Structural Performance. Biomacromolecules., 17 (4), 1358-1364. DOI: 10.1021/acs.biomac.6b00145